Mutation in yeast eIF4E (W75A) which affects its interaction

Mutation in yeast eIF4E (W75A) which affects its interaction with p20 or a knockout strain of p20 do not show a notable decrease in these properties. This is opposed to previously published data describing loss of pseudohyphenation in a diploid Dimethylenastron supplier homozygous Dp20 knockout strain [8]. We don’t have an explanation for these contradicting data. We conclude that the presence or absence of p20 is a less decisive factor for adhesive properties of yeast strains such as those examined in this work. This does not exclude that eIF4E-p20 interaction might modulate the translational rate of certain 25033180 genes required for adhesive properties [32]. As shown in this paper, in the yeast S. cerevisiae cap-dependent translation plays an important role for adhesion (to solid phases) ofhaploids and helps to trigger the differential program for pseudohyphenation upon nutritional starvation of diploids. This seems to contradict previous reports indicating the importance of cap-independent translation for proper expression of proteins involved in such processes. As an explanation, we would like to propose that signalling induced by nutritional starvation and allowing for cap-independent translation [16] is required for such differentiation processes. Once that such programs have been triggered, cap-dependent translation will still be required to allow for proper expression of e.g. housekeeping genes. Inhibition of adhesion can also be observed when elongation of translation is partially inhibited by adding to the medium limiting concentrations of cycloheximide (20?0 ng/ml) which to not impede growth of yeast strains used in this work (see Table S2; results not shown). This observation confirms a previous report [13] and allows for the more general conclusion that adhesion properties of yeast cells are rather sensitive to inhibition of protein synthesis. Adhesion plays also an important role in cancer metastasis and mammalian eIF4E and eIF4E-BPs have been shown to be involved via the mTOR pathway (for a review, see [33]). Adhesion and invasion require the proper expression of certain mRNAs and we would like to anticipate that beside evident differences between eukaryotic microorganisms and mammalian cells there will be common features in the way how cap-dependent translation is modulated to enhance or repress the expression of certain mRNAs involved in such processes. A careful analysis of the influence of mutants such as those described in this paper on gene expression patterns of haploid and diploid yeast strains will allow to further approach these questions.Supporting InformationFigure S1 Temperature sensitivity of eIF4E mutants. Serial 1:10 dilutions of all haploid eIF4E mutants were plated out and incubated on YPD at 30u or 35uC for 2 days, at 37uC for 3 days. (DOCX)NMR structure of yeast eIF4E in complex with m7GDP. Residues in the cap-binding site of eIF4E are displayed. E103, E105, D106 and E107 are marked in red, W104 in yellow and W75 in white, the backbone protein is displayed in yellow (PDB file – 1AP8). m7GDP is shown in blue, indicated are the positions of the positively charged 7-methyl imino group and the negatively charged phosphate groups. (DOCX)Figure S2 Figure S3 eIF4F knockouts Dtif3 and Benzocaine cost Dtif4631 loose adhesion and pseudohyphenation. (A) Adhesion of haploid Dtif1, Dtif2, Dtif3, Dtif4631 and Dtif4632 deletion mutants in comparison to wt. Plates were incubated at 30u or 35uC for 2 days, then washed under a gentle stream of water. (B) Pseudohyphena.Mutation in yeast eIF4E (W75A) which affects its interaction with p20 or a knockout strain of p20 do not show a notable decrease in these properties. This is opposed to previously published data describing loss of pseudohyphenation in a diploid homozygous Dp20 knockout strain [8]. We don’t have an explanation for these contradicting data. We conclude that the presence or absence of p20 is a less decisive factor for adhesive properties of yeast strains such as those examined in this work. This does not exclude that eIF4E-p20 interaction might modulate the translational rate of certain 25033180 genes required for adhesive properties [32]. As shown in this paper, in the yeast S. cerevisiae cap-dependent translation plays an important role for adhesion (to solid phases) ofhaploids and helps to trigger the differential program for pseudohyphenation upon nutritional starvation of diploids. This seems to contradict previous reports indicating the importance of cap-independent translation for proper expression of proteins involved in such processes. As an explanation, we would like to propose that signalling induced by nutritional starvation and allowing for cap-independent translation [16] is required for such differentiation processes. Once that such programs have been triggered, cap-dependent translation will still be required to allow for proper expression of e.g. housekeeping genes. Inhibition of adhesion can also be observed when elongation of translation is partially inhibited by adding to the medium limiting concentrations of cycloheximide (20?0 ng/ml) which to not impede growth of yeast strains used in this work (see Table S2; results not shown). This observation confirms a previous report [13] and allows for the more general conclusion that adhesion properties of yeast cells are rather sensitive to inhibition of protein synthesis. Adhesion plays also an important role in cancer metastasis and mammalian eIF4E and eIF4E-BPs have been shown to be involved via the mTOR pathway (for a review, see [33]). Adhesion and invasion require the proper expression of certain mRNAs and we would like to anticipate that beside evident differences between eukaryotic microorganisms and mammalian cells there will be common features in the way how cap-dependent translation is modulated to enhance or repress the expression of certain mRNAs involved in such processes. A careful analysis of the influence of mutants such as those described in this paper on gene expression patterns of haploid and diploid yeast strains will allow to further approach these questions.Supporting InformationFigure S1 Temperature sensitivity of eIF4E mutants. Serial 1:10 dilutions of all haploid eIF4E mutants were plated out and incubated on YPD at 30u or 35uC for 2 days, at 37uC for 3 days. (DOCX)NMR structure of yeast eIF4E in complex with m7GDP. Residues in the cap-binding site of eIF4E are displayed. E103, E105, D106 and E107 are marked in red, W104 in yellow and W75 in white, the backbone protein is displayed in yellow (PDB file – 1AP8). m7GDP is shown in blue, indicated are the positions of the positively charged 7-methyl imino group and the negatively charged phosphate groups. (DOCX)Figure S2 Figure S3 eIF4F knockouts Dtif3 and Dtif4631 loose adhesion and pseudohyphenation. (A) Adhesion of haploid Dtif1, Dtif2, Dtif3, Dtif4631 and Dtif4632 deletion mutants in comparison to wt. Plates were incubated at 30u or 35uC for 2 days, then washed under a gentle stream of water. (B) Pseudohyphena.