Tor region (SOA; Figures A,B),but few buy IMR-1 Terminals were identified within the oculomotor nucleus (III),as reported recently (Bohlen et al. A pretty dense terminal field was present inside the contralateral cMRF (Figures A,which may perhaps underlie the presence of an inhibitory off direction in tonically active cMRF neurons (Waitzman et al. Far more caudally,an comprehensive terminal field was discovered inside the SC (Figures C. Terminals were distributed to each sides,and were densest inside intermediate gray layer (SGI),in agreement with previous reports (Zhou et al. Wang et al. Labeled fibers extended caudally from the cMRF injection website and terminated densely and bilaterally within the cuneiform nucleus (Cun; Figure E). In the pons,labeled terminals have been discovered all through the PRF,which includes each its medially positioned magnocellular division and,to a lesser extent,in the laterally situated parvocellular division. These terminals distributed to each sides,with an ipsilateral predominance (Figures B. Around the ipsilateral side,a lot of fibers coursed diagonally,dorsolateral to ventromedial in the rostral end with the PRF (Figures B,ahead of taking up a medial location. Around the contralateral side,labeled axons that had decussated beneath the rostral pole of III took up a position just off the midline,beneath the brachium conjunctivum (Figures A,B),and maintained this position,operating just lateral to the raphe nuclei by way of the pons and into the medulla. They terminated extensively within the nucleus reticularis tegmenti pontis (nRTP; Figure D). Labeled terminals had been densest within the rostral,ipsilateral PRF (Figures C,D). A lot more caudally,the ipsilateral PRF terminals were concentrated medially,ventral and rostral to the abducens nucleus,where the PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/19957035 horizontal gaze center is positioned (Figures E. Nevertheless,scattered terminations were also present all through the parvocellular regions discovered laterally within the PRF. On the contralateral side,there have been fewer terminal arbors and they tended to be concentrated medially inside the PPRF (Figures D. This trend becomes additional evident as 1 precedes caudally. Many labeled terminals had been present on the midline in the nucleus raphe interpositus (RIP),exactly where the omnipause neurons lie (Figures C,as has been reported previously (Wang et al. Ultimately,BDA labeled terminals had been present within the parabrachial nuclei (PB),mainly ipsilaterally (Figure E). In the medulla,labeled cMRF terminals continued to target the reticular formation bilaterally (Figures G,but much more have been found ipsilaterally,as previously described (Perkins et al. Within the rostral medulla (Figure H),terminals were present dorsally within the medullary reticular formation (MdRF),where the IBN component of horizontal gaze center is situated. The vast majority of MdRF terminals had been situated dorsal to the inferior olive (IO),especially at far more caudal levels (Figures G. Terminals were rather evident ipsilaterally,inside the medial accessory nucleus of the IO (Figure I). Caudal for the area illustrated,the quantity of labeled terminals dropped off drastically,to ensure that only a compact variety of labeled terminals were present within the cervical spinal cord (for particulars,see Warren et al. Perkins et al. A additional detailed example with the pattern of terminal labeling in the level of the abducens nucleus is presented in Figure . In this case,the BDA injection was slightly larger than theFrontiers in Neuroanatomy www.frontiersin.orgApril Volume ArticleWang et al.A cMRF Tectoreticuloreticular PathwayFIGURE Distribution of central mesencephalic r.