E most effective response was accomplished in 19 individuals (25.six ) and no response to therapy with disease progression was seen in eight patients (10.8 ). Clinical benefit (CR or PR or SD for no less than 6 months) was accomplished in 55 sufferers (74.three ). Response could not be clearly identified in five individuals, in whom the disease metastasized predominantly to the skeleton. Median followup time was 41 months. Disease progression was documented in 64 (86.5 ) individuals. Median TTP for the complete group was 9.two months (range from 1.3 to 56.two months), median OSt was 20.1 months (variety 1.three to 68.3 months) and OSm was 29.8 months (range from 1.75 to 83 months). The majority of tumours were invasive ductal carcinomas (59 individuals, 79.7 ) and scored as grade three (59.five ), 32 patients had an ERpositive tumour (43.2 ), 20 patientsTable I. Patient and tumour characteristics. Characteristic Age (years): median 54 (range 3274) 60 60 Performance status 0 1 two NA Histology Ductal Lobular Mixed Other Tumour grade G1 G2 G3 UN ER status Optimistic Adverse NA PgR status Good Adverse NA Position of trastuzumab in palliative therapy 1. line two. line three. line Combination of trastuzumab with cytostatics Paclitaxel Glycosyltransferase Inhibitors MedChemExpress Docetaxel Vinorelbine CBDCA paclitaxel No cytostatics (trastuzumab monotherapy) Best response to therapy CR PR SD PD NA Sufferers no.57 17 25 39 5 5 59 7 two 6 1 13 44 16 32 40 2 20 4977.0 23.0 33.7 52.7 six.8 6.8 79.7 9.5 2.7 8.1 1.3 17.six 59.five 21.6 43.two 54.1 two.7 27.0 66.2 six.44 2359.5 31.1 9.39 18 eight 652.7 24.three 10.8 8.1 four.9 33 19 812.2 44.6 25.six 10.eight six.CBDCA, carboplatin; CR, full remission; ER, estrogen receptor; NA, not assessed; no., quantity; PD, progressive illness; PgR, progesterone receptor; PR, partial remission; SD, stable illness;. UN, unascertained (which includes a group of tumours exactly where grading was G23). All tumours were IHC three orand FISHpositive.GRELL et al: Akt EXPRESSION IN PREDICTING THE RESPONSE TO TRASTUZUMABFigure 1. Examples of IHC assessment of Akt expression and compartmentalization.had a PgRpositive tumour (27.0 ) and 35 had an ER andor PgRpositive tumour (47.three ). Akt expression and compartmentalization in breast cancer. Seventyfour key tumour samples were analyzed for Akt1, Akt2, pAkt Thr308 and pAkt Ser408 expression and scored as described above. Seventeen tumours (23.0 ) had been adverse on Akt1 staining, 46 (62.1 ) have been Akt1 weak optimistic and 9 (12.2 ) had been powerful good, outcomes for two tumours were not interpretable. There were no Akt2negative tumours, 45 (60.8 ) samples have been weak positive (Fig. 1A) and 26 (35.1 ) were powerful good on Akt2 Nisoxetine Monoamine Transporter staining (Fig. 1B), outcomes for three tumours have been not interpretable. For phosphorylated Akt, cytoplasmic and nuclear fractions were assessed separately. Ten tumours (13.five ) had been unfavorable on any pAkt Thr308 staining, 22 tumours (29.7 ) were optimistic on cytoplasmic staining only (Fig. 1C) and 38 (51.4 ) were optimistic on each nuclear and cytoplasmic (nc) staining (Fig. 1D), 4 instances (5.four ) could not be assessed. For pAkt Ser473 staining, five situations had been negative, 16 instances (21.6 ) were optimistic on cytoplasmic staining only and 49 (66.2 ) had been good on each nuclear and cytoplasmic staining, four cases (5.four ) couldn’t be interpreted. Akt expression final results are summarized in Table II. We located no correlation in between expression of Akt1, Akt2 or activated types of Akt and expression of estrogen or progesterone receptors or tumour grading. Correlation of Akt expression and compartmentalization with time for you to progression. For patien.