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W in Chironomus fed with TetraMinbut decreased the proportions of EPA and ARA in Chironomus fed with Microcystis (ESM Tables S1 and S2). In Micro20 and Micro25 diet therapies, warming IFN-beta Protein medchemexpress drastically decreased the proportion of ARA in Chironomus larvae from about 7.2.8 w to five.2.eight w when the experimental temperature increased from 20 C to 25 C (ESM Table S1). Inside the Micro20 treatment, the EPA w in Chironomus declined from 4.0 w to 3.5 w , however the decline was not statistically significant (p = 0.098, ESM Table S2), whereas in the Micro25 therapy the temperatureinduced decline in EPA from 3.two w to two.three w in Chironomus was statistically important (ESM Table S2).Biomolecules 2021, 11,5 ofTable 1. Major fatty acids and sum of n3 and n6 PUFA expressed as w of total fatty acids, as well as the n3/n6 ratio in diets: Micro20, Micro25, and TetraMinfish flakes (quantity of samples n = three for all diets). The presented fatty acids accounted for much more than 99 of all fatty acids in Microcystis diets (Micro20 and Micro25), and much more than 92 of all fatty acid in TetraMin. The reduce percentage in TetraMinis as a result of exclusion of longchain saturated (C20 LCSFA) and monounsaturated fatty acids (C20 LCMUFA) from the table, which are prevalent in TetraMinbut not in Microcystis, see also Strandberg et al. [16]. Fatty Acid 14:0 i15:0 15:0 16:0 16:1n9 16:1n7 18:0 18:1n9 18:1n7 18:2n6 18:3n6 18:3n3 18:4n3 20:2n6 20:3n6 20:4n6 20:3n3 20:4n3 20:5n3 22:5n3 22:6n3 Sum n6 PUFA Sum n3 PUFA n3/n6 Micro20 Imply 0.40 0.28 0.08 44.19 0.47 1.40 four.25 1.20 three.00 3.04 9.40 10.87 18.04 0.00 0.35 0.00 0.84 1.93 0.00 0.00 0.00 12.79 31.69 2.48 SD 0.10 0.06 0.03 1.40 0.02 0.13 1.86 0.13 0.80 0.13 0.35 0.44 1.02 0.00 0.20 0.00 0.37 0.50 0.00 0.00 0.00 0.18 0.61 0.05 Micro25 Mean 0.56 0.87 0.29 46.23 0.24 1.64 6.38 1.92 five.55 6.79 16.95 five.02 6.05 0.00 0.71 0.00 0.19 0.21 0.00 0.00 0.00 24.45 11.47 0.47 SD 0.16 0.23 0.08 three.93 0.06 0.09 3.31 0.39 0.70 0.31 1.13 0.92 1.15 0.00 0.69 0.00 0.17 0.16 0.00 0.00 0.00 0.19 2.11 0.08 TetraMinMean 2.82 0.05 0.21 15.08 0.08 2.63 11.75 24.86 two.40 22.52 0.01 3.46 0.34 0.19 0.01 0.14 0.02 0.09 two.82 0.32 2.92 22.87 9.96 0.44 SD 0.00 0.00 0.01 0.40 0.01 0.01 0.17 0.25 0.04 0.19 0.00 0.09 0.02 0.03 0.00 0.02 0.02 0.02 0.15 0.03 0.16 0.15 0.13 0.Table 2. Twoway ANOVA GM-CSF Protein Human summary table for EPA w and ARA w in Chironomus riparius across diet and temperature treatment options. Variable EPA w Source Diet plan Temperature Diet program Temperature interaction Inside group Total Diet plan Temperature Diet plan Temperature interaction Within group Total df two 1 2 23 28 two 1 2 23 28 SS 44.218 1.148 1.529 5.577 53.367 155.027 12.715 six.598 14.783 191.348 MS 22.109 1.148 0.764 0.242 77.513 12.715 3.299 0.643 F 91.176 4.733 3.152 p 0.001 0.040 0.062 Impact Size (Eta Squared) 0.829 0.022 0.ARA w120.595 19.782 5.0.001 0.001 0.0.810 0.066 0.Biomolecules 2021, 11, xBiomolecules 2021, 11,6 of6 ofFigure 2. Estimated marginal implies for (A) EPA w and (B) ARA w in Chironomus fed with either Micro20, Micro25, or TetraMin(manage) and maintained at two distinctive experimental temperatures: 20 C and 25 C. Error bars represent 95 TetraMin(manage) and maintained at two unique experimental temperatures: 20 and 25 . Error bars represent 95 self-assurance intervals. The imply EPA w values differed between the Microcystis diet remedies (Micro20 and Micro25). The self-assurance intervals. The imply EPA w values differed between the Microcystis diet plan treatments (Micro20 and Micro25). imply EPA w decreased with inc.

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