S. Grass-fed beef has been gaining recognition simply because it supports trends towards much more substantial land use and delivers well being benefits as a result of its favorable fatty acid (FA) profile, like elevated proportions of conjugated linoleic acid (CLA) and n-3 FAs [1,2]. Fat can exert each good and damaging effects on human overall health, based on its FA composition . Fat deposited in several regions in the carcass can have unique FA profiles . Intramuscular fat content of cattle is influenced by variables such as breed, sex, age and housing method but also the person genetic background of an animal . Lately, it has been shown that the ELOVL6 gene regulates the lipid metabolism and encodes a essential protein that participates in lipogenesis by catalyzing the elongation of monounsaturated and saturated fatty acids . The FA composition of adipose tissueCopyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This short article is an open access report distributed beneath the terms and circumstances of your Creative Commons Attribution (CC BY) license (licenses/by/ 4.0/).animals 2021, 11, 3078. ten.3390/animdpi/journal/animalsAnimals 2021, 11,two ofis also determined by the nutritional regime of cattle plus the length from the fattening period [13,14]. Fattening techniques differ from farm to farm, and fattening intensity is determined by on-farm feeding capability, Nemonapride Autophagy production profitability and market place demand. Regular and semi-intensive (SI) cattle production systems are most well-known in Poland. In comparison with intensive (I) production systems, meat made beneath SI systems has low levels of saturated FAs (SFAs) and high concentrations of n-3 FAs and CLA [15,16]. The aim of this study was to evaluate the impact of two cattle production systems on the FA profiles of distinct fat depots. The analyzed production systems are widespread in Poland. Meat from Polish Holstein-Friesian (PHF) cattle and their crosses predominates on the domestic marketplace. 2. Materials and Techniques The study was performed upon the approval with the Ethics Committee from the Linoleoyl glycine Inhibitor University of Warmia and Mazury (decision No. 121/2010). The experimental supplies comprised 16 crossbred bulls created by crossing PHF cows with Limousin (LM) bulls. Manage fattening began at 7 months of age, immediately after a 1-month adaptation period, and it lasted until 18 months of age. The animals have been divided into two groups, and they have been fattened semi-intensively (SI) and intensively (I). The Total Mixed Ration (TMR), composed of grass silage and concentrate (triticale, rapeseed meal (RSM), premix), was offered ad libitum. Two kinds of concentrate with unique protein content material were utilised (Table 1). The concentrates have been formulated according to the amounts of protein digested within the little intestine, PDIN and PDIE . Depending on the production program, the ratio of concentrate to grass silage (on a dry matter basis) was 25:75 (SI) and 40:60 (I) (Table two). The ratios were adjusted every single 4 weeks depending on regular silage analysis. Bulls with BW under 300 kg received TMR containing concentrate I (25 RSM), and bulls with BW above 300 kg received TMR containing concentrate II (19 RSM).Table 1. Chemical composition and nutritional value of experimental diets (mean SD). Specification Triticale (g/kg) Rapeseed meal (g/kg) Dry matter In Dry matter [g/kg] Organic matter Crude protein NDF 1 ADF 2 DOMD three UFV four PDIN 5 PDIE six Silage n=9 Triticale n=1 Rapeseed Meal n=1 Concentrate I n=7 710 250 883.9 7.1 932 13.