Ies, and related photoautotrophs. Environmental situations at the variety locality: Discharge (L s-1): 3.five, Temperature ( C): five.3, conductivity ( cm-1): 62, alkalinity ( q L-1): 311, pH: six.6, nitrate (mg L-1): 1.2, TP ( L-1): 7 (see  for a lot more particulars). As concerns photoautotrophs, in this very shaded source the competitive balance amongst big groups (algae, lichens, bryophytes, and vascular plants) is clearly favorable for the mosses, which cover nearly all of the lithic substrata [dominance of Brachythecium rivulare W.P. Schimper, both submerged and, in big portions, emerged, and also a certain relevance of Plagiomnium undulatum (Hedw.) T.J. Kop. and Rhizomnium punctatum (Hook.) T.J. Kop.]. Vascular plants are usually not abundant (as cover), and Adenostyles glabra (Miller) DC. and Saxifraga rotundifolia L. might be talked about among them. When it comes to cover, bryophytes are followed by lichens. These contain two species which are seldom reported in Italy: Verrucaria madida Orange, an amphibious species in regularly flooded web sites on AZD1656 site siliceous rocks, usually in association with other aquatic lichens and bryophytes, and Verrucaria aquatilis Mudd., typical each in springs and along streams, in circumstances of perennial/frequent submersion. Benthic macroalgae are uncommon and mainly represented by the red freshwater alga Hildenbrandia rivularis (Liebmann) J. Agardh, that is characteristic of shaded springs with well-buffered waters and medium-high conductivity. The main co-occurring diatom species at the type locality (at least five relative abundance in certainly one of the slides): Achnanthidium minutissimum sp. gr., Amphora inariensis Krammer, Amphora indistincta Levkov, Brachysira exilis (K z.) Round and D.G.Mann Cocconeis pseu-Diversity 2021, 13,8 ofDiversity 2021, 13,Sulphates (mg L-1) Cl-1 (mg L-1) TN ( L-1) SRP ( L-1) Mn ( L-1) Zn ( L-1)dolineata (Geitler) Lange-Bert., Gomphonema elegantissimum E.Reichardt and Lange-Bert., Humidophila perpusilla (Grunow) Lowe, Kociolek, J.R.Johansen, Van de Vijver, Lange-Bert. 9 of 20 and Kopalov Planothidium angustilanceolatum sp. nov., P. frequentissimum (Lange-Bert.) Lange-Bert., P. lanceolatum, Sordarin Autophagy Psammothidium grischunum Bukht. and Round. Ecology (Table 1, Figure 3a). With reference to temperature, E. crassiminor has a reduced optimum weighted typical than E. minor (Table 1); consistently, E. crassiminor -11 5.1 1.four 11.5 two.2 1.4 four.three -7.39 1.45 ten also seems 0.4prefer sites0.2 to which are additional shaded (Figure 3b). As concerns pH2.28 10-5 (Figure 3e), 1.7 0.8 0.two 0.8 4.53 interestingly, E. crassiminor1272 seems to favor circumneutral values while E. minor happens 340 0 649 0 1272 four.29 7.69 10-5 at slightly acidic ones. E. crassiminor includes a greater weighted average -2.25 for sulphates whilst 2 0 6 2 0 three two.69 10-2 E. minor includes a greater optimum for manganese (Table 1). In particular, with reference to 0.6 0.2 0.two 9.eight 7.five 7.five four.95 two.59 10-5 nitrogen, E. crassiminor seems to be related with additional strict oligotrophy than E. minor. 101 0 40 37 0 107 -2.12 3.56 10-Figure 3. (a). Box and whisker plots displaying the ecological preferences of Eunotia crassiminor as compared to E. minor. Figure 3. (a). Box and whisker plots displaying the ecological preferences of Eunotia crassiminor as in comparison to E. minor. Only environmental factors/parameters for which statistically considerable differences could possibly be located are shown. Only environmental factors/parameters for which statistically substantial variations could possibly be located are shown.Taxonomic comments.