Hiocyanate, it truly is necessary to separate and enrich thiocyanate from raw milk, so that the interference of other components of raw milk in the detection procedure can be excluded. Amongst the available procedures for thiocyanate enrichment, solidphase extraction procedures have largely been studied. Al-Saidi et al.  established a headspace sorptive solid-phase microextraction (HS-SPME) technique for the extraction of thiocyanate and cyanide. Under the optimized circumstances, the LOD and LOQ were 0.34 and 1.two mmol/L, respectively. Da Silva et al.  separated thiocyanates GS-626510 supplier applying capillary electrophoresis. The ranges of LOD and LOQ have been 0.03.04 and 0.05.07 mg/L. Though the IQP-0528 manufacturer electromigration technique is affordable and environmentally friendly, it needs a high level of professionalism in the operator and is just not easily promoted in factories. Lu et al.  extracted thiocyanate ions within the aqueous phase with complexes of Hg2 into methyl isobutyl ketone and the LOD was 1.33 ng/mL. Having said that, the presently reported procedures for thiocyanate determination endure from the disadvantages of cumbersome pretreatment, low sensitivity, lots of influencing elements, and high cost. For that reason, a more rapid, sensitive, easy, and economical strategy for the enrichment and detection of thiocyanate in raw milk is needed. An aqueous two-phase technique (ATPS) is formed primarily by the partitioning of two immiscible options. In some fields, ATPS is often a novel option strategy to conventional solvent extraction. ATPS has successfully been made use of for the extraction and purification of peptides , polysaccharides , enzymes , heavy metals , proteins , amino acids , cells , and cytochromes , and has also been utilised for the separation and enrichment of neutral, anionic, and cationic ions . In current years, some novel, low-cost, and efficient ATPSs have appeared that are unique from standard polymer/salt and ionic liquid/salt systems. These ATPSs have received growing consideration as a consequence of high extraction efficiency, speedy phase separation, low viscosity, mild environmental effects, and recyclability, including alcohol/inorganic salt systems , propanol/inorganic salt systems , acetonitrile/inorganic salt systems , and so on. These ATPSs enable the recovery of modest organic molecules in the ATPSs by evaporation and crystallization, making it simple to separate the extract from the wealthy organic phase, thus reducing the price of extraction and enrichment, and simplifying the subsequent production approach for effortless application in downstream production . In analytical applications, such systems very easily exclude the probable interference of background in the determination of target substances. Since the introduction of ion chromatography (IC) by Shapiro et al.  in 1975, the system has grow to be the preferred strategy for the determination of compact inorganic and organic ions. Thienpont et al.  reported that IC could decide the total sodium and potassium concentrations in human serum. Charles et al.  proposed a strategy of ion chromatography-mass spectrometry (IC-MS) to ascertain bromate ions in water. Fernandes et al.  applied IC combined with UV detection to detect four bisphosphonates in pharmaceuticals or bulk components. Now, the determination of thiocyanate in the industry is largely primarily based on the spectrophotometric strategy, which is primarily based on the principle that SCN- can produce blood-red iron thiocyanate complicated ion ([Fe(SCN)n]m) with Fe.