Extensively available instrument to gain in-depth insights into the molecular signatures of EV sub-populations in

Extensively available instrument to gain in-depth insights into the molecular signatures of EV sub-populations in the single EV level. Targeting a number of markers enormously improves differentiation of EV sub-populations. The high simplicity of our approach and its excellent adaptivity to clinical labs is going to be highly effective for screening for powerful EV markers for liquid biopsy applications. Funding: NIH-NCIISEV2019 ABSTRACT BOOKSymposium Session 27: Non-mammalian EVs Chairs: Richard Ferrero; J. Max Silverman Area: Level B1, Hall B 16:007:OS27.Extracellular vesicles launched by commensal Lactobacillus suppress HIV-1 infection Rogers A. Nahui Palominoa, Christophe Vanpouillea, Peter Backlundb, Carola Parolinc, Luca Laghid, Beatrice Vitalic and Leonid Margolisaa Part of Intercellular Interaction, Nectin-3/CD113 Proteins Biological Activity Eunice Kennedy Shriver Nationwide Institute of Child CD300c Proteins Formulation Health and Human Development, National Institutes of Well being, Bethesda, MD, USA; bBiomedical Mass Spectrometry Facility, Eunice Kennedy Shriver Nationwide Institute of Child Well being and Human Advancement, National Institutes of Health, Bethesda, MD, USA; c Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy; dCentre of Foodomics, Department of Agro-Food Science and Technology, University of Bologna, Bologna, Italyamino acids, alcohols, ketones and monosaccharides. Proteomic analysis showed the presence of many bacterial proteins in EVs that may be connected with all the anti-HIV effect. Summary/Conclusion: Our findings demonstrate that the protective effect of Lactobacillus against HIV transmission is, in component, mediated by EVs launched by these commensal bacteria. This discovering might cause new tactics to stop male-to-female sexual HIV transmission.OS27.Extracellular vesicles on the human gut microbiota: do you hear me host Anna Kaisanlahtia, Anatoliy Samoylenkob, Genevieve Bartb, Johanna Korvalaa, Annastiina Rytk enc, Artem Zhyvolozhnyic, Ilkka Miinalainenc, Leo Lahtid, Seppo Vainioe and Justus Reunanenfa Biocenter Oulu/Cancer and Translational Medication Analysis Unit, University of Oulu, Oulu, Finland; bUniversity of Oulu, Biocenter Oulu, Laboratory of developmental Biology, Oulu, Finland; cBiocenter Oulu, University of Oulu, Oulu, Finland; dDepartment of Mathematics and Statistics, University of Turku, Turku, Finland; eUniversity of Oulu, Biocenter Oulu, Laboratory of Developmental Biology, Oulu, Finland; f University of Oulu, Biocenter Oulu, Cancer and Translational Medication Analysis Unit, Oulu, FinlandIntroduction: The vaginal microbiota, typically dominated by Lactobacillus spp. plays a important part in preventing from many uro-pathogens’ infections, in particular from HIV-1. Not too long ago, we demonstrated that Lactobacillus of different strains inhibit HIV-1 replication in human cervico-vaginal and tonsillar tissues ex vivo delivering an experimental program to examine mechanisms of this phenomenon. A rising entire body of evidences propose that any kind of cells, such as bacteria talk to one another as a result of extracellular vesicles (EVs). Here, we investigated whether the protective anti-HIV impact of lactobacilli is mediated by EVs launched by these bacteria. Approaches: EVs had been isolated from 4 strains of Lactobacillus cultures, previously isolated from vaginas of healthy gals, by ultracentrifugation. Vesicles’ sizes and concentrations had been evaluated making use of NanoSight. Human cervico-vaginal and tonsillar tissues ex vivo, too as cell lines had been handled with Lactobacillus-deri.