F Nanotechnology Advanced Materials, Bar-Ilan University, Israel, Ramat Gan, USA; cSchool of Neurobiology,

F Nanotechnology Advanced Materials, Bar-Ilan University, Israel, Ramat Gan, USA; cSchool of Neurobiology, Biochemistry and Biophysics, Life sciences faculty, Tel Aviv University, Israel, Tel Aviv, Israel; dSacklar College of medicine, department of human genetics and biochemistry Tel Aviv University, Israel, Petah Tikva, Israel; eSacklar College of Medicine, Department of Human Genetics and Biochemistry Tel Aviv University, Israel, Petah Tikva, USA; fSagol College of neuroscience, Tel Aviv University, Israel. College of Neurobiology, Biochemistry and Biophysics, Life Sciences Faculty, Tel Aviv University, Israel, Tel Aviv, Israel; gSagol College of Neuroscience, Tel Aviv University,bISEV2019 ABSTRACT BOOKIsrael, Sacklar College of Medicine, Department of Human Genetics and Biochemistry Tel Aviv University, Israel, Tel Aviv, USAIntroduction: Although exosoemes happen to be identified to cross the blood rain barrier, their migration and homing skills within the brain remain unstudied. We’ve got not too long ago developed a strategy for longitudinal and quantitative in vivo neuroimaging of exosomes, determined by the superior visualization abilities of CT, combined with gold nanoparticles as labelling agents. Right here, we employed this method to track the migration and homing patterns of intranasally administrated exosomes derived from bone marrow mesenchymal stem cells (MSC-exo) in distinct brain pathologies, such as stroke, autism, Parkinson’s disease and Alzheimer’s disease. We discovered that MSC-exo particularly targeted and accumulated in pathologically-relevant murine models brains regions as much as 96 h post administration, whilst in wholesome controls they evacuated. The neuroinflammatory signal in pathological brains was very correlated with MSC-exo accumulation. Additionally, MSC-exo had been selectively uptaken by neuronal cells in the pathological regions. Procedures: Exosomes were extracted from human bone marrow mesenchymal stem cells. They have been loaded with glucose-conjugated gold nanoparticles and weregiven by means of intranasal administration to mice with diverse pathologies. All mice were scanned with CT 1, 24 and 96 h post administration. In addition, employing PKH26 MSC-exo had been labelled and had been visualized with complete brain florescence. Outcomes: Altogether, our Data suggests that MSC-exo present distinct neurodistribution which is pathologyspecific in each with the mice models visualized each in vivo and ex-vivo. In both the induced stroke and Parkinson’s models, the MSC-exo have been visualized mainly inside the damaged tissue (Striatum). In Alzheimer’s model, they had been visualized mostly within the hippocampus, and within the Autism mice model, they were visualized each within the prefrontal cortex and the cerebellum. Interestingly, in healthful mice the exosomes didn’t home to any precise location as well as the signal was lost 24 h post administration each in vivo and ex vivo. Inside the broken tissue, the MSC-exo were identified mainly inside the neurons and not in other cells. Summary/conclusion: Taken together, these findings can drastically market the application of exosomes for PD-L1/CD274 Proteins Formulation therapy and targeted drug delivery in numerous brain pathologies by way of intranasal administration.JOURNAL OF EXTRACELLULAR VESICLESSymposium Session 22: Novel Techniques of EV Evaluation Chairs: An Hendrix; John Nolan Place: Level B1, Hall A 16:308:OF22.Biolayer ICOS Proteins Molecular Weight interferometry extracellular vesicles (BLIEV) platform for liquid biopsy of ovarian cancer Tatu Rojalina, Randy Carneya and Kit LambaUC Davis, Davis, USA; bUniversity of California,.