Ical signals. The evidence shows that within populations of EVs, critical properties like morphology, composition

Ical signals. The evidence shows that within populations of EVs, critical properties like morphology, composition and content vary substantially. Therefore, measuring EV heterogeneity is paramount to our understanding of how EVs influence physiological and pathological functions of their target cells. Hence far, devising effective solutions for measuring EV heterogeneity remains a international challenge. Approaches: We present, for the first time, a study from the molecular and structural composition of person EVs, subpopulations of EVs and complete populations of EVs employing resonance enhanced atomic force microscope infrared spectroscopy (AFM-IR). This approach is labelfree, has ultra-high sensitivity and has the power to measure EV heterogeneity. EVs were isolated from placenta stem cells using ultrafiltrationFriday, 04 Mayand following additional purification using the extra size-exclusion chromatography column and each methods were compared. Outcomes: We demonstrated for the first time the possibility to characterise individual EV at nanoscale, EV populations and showed the critical variations in their composition depending on extraction protocols heterogeneity. Ultra-high resolution of AFM-IR that permits probing of a number of points on individual EVs is key to develop new extraction and separation protocols for EVs and to unlock their full therapeutic and diagnostic potential. Our method outperforms other approaches for vesicles characterization supplying unmatched resolution (single vesicle) and is “probe free”, thus it avoids bias and resolution limitations of molecular probes. Summary/Conclusion: The AFM-IR is advancing the EV field forward by revealing their molecular constituents and structures, at the same time as enabling purity assessment of EV preparations. The information presented within this study recommend AFM-IR can transform current protocols for interrogating EV composition and structures, and assessing EV purity. This nanoscale method is often developed into a highly effective screening tool for detecting particular EV “fingerprints” which can be associated with pathology by correlating the structural differences to biomarkers, addressing unmet clinical needs in illnesses where early diagnosis is important, as an example many sclerosis or cancer.resulting from (1) competitors involving capture and labeling antibody in TRFIA when exactly the same antibody is utilized, and (two) a non-linear connection involving refractive index-based and labeling-based detection. Our outcomes Anti-Mullerian Hormone Receptor Type 2 Proteins Source indicate that results of diverse quantitative phenotyping procedures have to be addressed with care. For that reason, we recommend to Death-Associated Protein Kinase 3 (DAPK3) Proteins Recombinant Proteins translate the results into typical antigen density on detected EVs to allow the comparison of outcomes. Funding: This function was supported by the Cancer-ID perspectief plan of NWO Applied and Engineering Sciences [Project #14197].OF12.Proximity assays for detection and characterization of exosomes Ehsan Manouchehri; Alireza Azimi; Qiujin Shen; Masood KamaliMoghaddam Division of Immunology, Genetics and Pathology, IGP Uppsala University, Uppsala, SwedenOF12.Membrane protein quantification on extracellular vesicles by surface plasmon resonance imaging and time-resolved fluorescence immunoassay Elmar Gool1; Frank A.W Coumans2; Janne Leivo3; Mirella Vredenbregt – van den Berg4; Auguste Sturk5; Ton G. van Leeuwen2; Rienk Nieuwland5; Guido W. Jenster4 Department of Biomedical Physics and Engineering (BMEP) Department of Clinical Chemistry (LEKC) Academic Medical Center, Amsterdam, The Netherla.