Pigment via thin layer chromatography (TLC), Fouriertransform infrared spectroscopy (FT-IR), and
Pigment by means of thin layer chromatography (TLC), Fouriertransform infrared spectroscopy (FT-IR), and proton nuclear magnetic resonance (1 Htransform infrared spectroscopy (FT-IR), and proton nuclear magnetic resonance (1HNMR) analyses revealed the presence of antimicrobial pigment rodiginine derivatives NMR) analyses revealed the presence of antimicrobial pigment rodiginine derivatives in Streptomyces sp. BSE6.1 [25]. On the other hand, the genome evaluation of strain BSE6.1 reveals the in Streptomyces sp. BSE6.1 [25]. Nonetheless, the genome analysis of strain BSE6.1 reveals the presence of an undecylprodigiosin gene cluster that is accountable undecylprodigiosin presence of an undecylprodigiosin gene cluster that is accountable forfor undecylprodigiproduction. Amylases Gene ID Therefore, the the red red fraction of Streptomyces strain BSE6.1 [25] to be osin production. Hence,otherotherfraction of Streptomyces strain BSE6.1 [25] is however is however 13 elucidated and and identified by means of LC-MS, 13C NMR, HSQC, HMBC, and COSY data to become elucidated identified via LC-MS, C NMR, HSQC, HMBC, and COSY data to confirm the production of undecylprodigiosin or related derivatives. to confirm the production of undecylprodigiosin or connected derivatives. Prior studies reported that Streptomyces longisporus, Streptomyces spectabilis [7,57], Prior studies reported that Streptomyces longisporus, Streptomyces spectabilis [7,57], and Streptomyces variegatus generate prodigiosin [16] (Table 1). Nonetheless, some strains of and Streptomyces variegatus make prodigiosin [16] (Table 1). Nonetheless, some strains of Streptomyces coelicolor produce either undecylprodigiosin [17,20,58] or possibly a mixture of prodigStreptomyces coelicolor generate either undecylprodigiosin [17,20,58] or even a mixture of prodiinine derivatives [59] (Table 1). Related to S. coelicolor [17,20,58,59], the very first fraction of ginine derivatives [59] (Table 1). Comparable to S. coelicolor [17,20,58,59], the initial fraction of red pigment eluted from Streptomyces strain BSE6.1 by way of TLC revealed the presence red pigment eluted from Streptomyces strain BSE6.1 by means of TLC revealed the presence of of methyl-3-propyl prodiginine and 2-methyl-3-butyl prodiginine in mass spectrometry methyl-3-propyl prodiginine and 2-methyl-3-butyl prodiginine in mass spectrometry evaluation but identified it as prodigiosin in 1 H NMR analysis [25]. Methyl-3-propyl prodiganalysis but identified it as prodigiosin in 1H NMR analysis [25]. Methyl-3-propyl prodiinine and 2-methyl-3-butyl prodiginine had been also identified in actinomycetes [60], nonginine and 2-methyl-3-butyl prodiginine were also identified in actinomycetes [60], nonactinomycetes bacteria like Apical Sodium-Dependent Bile Acid Transporter manufacturer Pseudoalteromonas rubra [61], and Serratia marcescens [62]. actinomycetes bacteria for example Pseudoalteromonas rubra [61], and Serratia marcescens [62]. These studies recommend that some strains of Streptomyces produce either prodigiosin or These studies recommend that some strains of Streptomycesof prodiginine analogs. undecylprodigiosin, whereas some generate a mixture generate either prodigiosin or undecylprodigiosin, whereas someof strain BSE6.1 created a total of 7,528,288 reads. AssemWhole-genome sequencing make a mixture of prodiginine analogs. bling these raw reads resulted within a single scaffold of 8.02 Mb with no extra-chromosomal content material. Annotating the assembled genome of strain BSE6.1 indicated the presence of at least 7157 protein-coding genes, 82 tRNA coding genes, 3 rRNA coding genes, and.
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