Yanate ABC transporter Chaperonin (GroEL) DNA-directed RNA polymerase beta subunit (RpoB) 50S ribosomal protein L4

Yanate ABC transporter Chaperonin (GroEL) DNA-directed RNA polymerase beta subunit (RpoB) 50S ribosomal protein L4 (Rpl4,RplD) Glutamine synthetase, glutamate-ammonia ligase (GlnA) 1 PO4 3- 6.six 1.two two.four 0.7 5.7 0.1 12.1 1.three 3.3 0.6 38.2 11.7 three.1 1.8 four.8 1.5 5.5 2.5 55.8 15.five 4.0 0.4 five.six 6.five 20.0 four.5 65 PO4 3- 14.five 0.4 five.1 1.three 11.9 2.1 25.1 1.2 6.9 2.6 78.7 9.4 six.4 0.eight 9.eight two.1 11.1 two.7 112.0 0.1 8.1 1.6 11.1 1.five 39.six 1.six Low/high fold change -2.2 -2.1 -2.1 -2.1 -2.1 -2.1 -2.1 -2.1 -2.0 -2.0 -2.0 -2.0 -2.Units are spectral counts. Arranged in highest to lowest fold adjust Zn-high PO4 3- , than Zn-low PO4 3- . +, fold higher than Zn-high PO4 3- ; -, fold less than Zn-high PO4 3- ; ukn, unknown; gi, genetic data processing; re, DNA replication and repair; m, metabolism; cb, carbohydrate metabolism; as, amino sugar metabolism; rg, regulatory function; t, translation; nu, nucleic acid metabolism; py, pyrimidine metabolism; a, amino acid metabolism; l, lipid metabolism; ps, HDAC6 Inhibitor Storage & Stability photosynthesis; abc, ABC transporter; s, sulfur metabolism; v, vitamin metabolism; po, porphyrin metabolism; chl, chlorophyll metabolism; tca, citrate cycle; e, energy metabolism; c, carbon fixation; g, glutathione metabolism; ei, environmental information and facts processing; si, signaling; p, phosphorus metabolism; f, protein folding; pu, purine biosynthesis; n, nitrogen metabolism.Table three | Relative protein abundances in between added (+ 4.four pM Cd2+ ) and no added Cd remedies for proteins two-fold or higher differentially abundant (phosphate replete and scarce Zn for both). SYNW ID KEGG Function Protein +4.4 pM Cd2+ No added Cd2+ Cd2+ /no Cd2+ fold alter +4.9 +3.three +3.2 +2.5 +2.0 -3.6 -3.4 -2.9 -2.three -2.1 Fisher test P-value0908 0670 0827 2191 0082 1118 0405 2139 1953ukn ukn ukn ps m,v,r m,cb m,nu,pu,a gi,t ukn,l m,cb,tca,e,cHSP90 Inhibitor review Hypothetical protein Hypothetical protein Hypothetical protein Photosystem II complex extrinsic protein precursor (PsuB) Riboflavin synthase subunit beta (RibH) Glucose-1-phosphate adenylyltransferase (Agp, GlgC) fumarate lyase: adenylosuccinate lyase (PurB) 30S ribosomal protein S10 (Rps10, RpsJ) Putative glycerol kinase Aconitate hydratase (AcnB)six.2 0.5 7 0.four .2 11.0 2.four 13.8 0.2 eight.six 2.four 1.5 0.7 1.9+0.1 1.9 0.1 2.four 2.1 two.four 0.1.3 0.6 2.2 1.9 three.4 0.1 five.5 1.7 four.three 0.1 5.five 0.5 six.four 0.8 5.five 1.7 5.six two.0 5.1 1.95 (0.01) 95 (0.0048) 95 (0.0016) 95 (0.0016) 95 (0.047) 95 (0.019) 95 (0.041) 0 (0.09) 0 (0.15) 0 (0.21)Units are spectral counts. Arranged in highest to lowest fold alter four.four pM Cd2+ vs. manage (no Zn and no added Cd). +, fold higher than control; -, fold less than control; ukn, unknown; ps, photosynthesis; m, metabolism; v, vitamin metabolism; r, riboflavin metabolism; cb, carbohydrate metabolism; nu, nucleic acid metabolism; pu, purine metabolism; a, amino acid metabolism; gi, genetic data processing; t, translation; l, lipid metabolism; tca, citrate cycle; e, power metabolism; c, carbon fixation, within this case reductive glyoxylate cycle.The response to a mixture of Zn and PO4 3- scarcity was significantly various, especially for proteins in higher abundance under PO4 3- scarcity (Figure 5A; Table 2). None in the proteins have been precisely the same because the Tetu et al. (2009) transcriptomestudy. Most notable was the influence on two crucial PO4 3- acquisition proteins: the alkaline phosphatase and phosphate transporter described above (Figures 7B,C), which were only modestly affected by PO4 3- limitation under Zn scarcity. InFrontiers in Mi.