Product Name :
Mouse anti-Cytokeratin 18 Monoclonal Antibody
Synonym :
CYK18; Keratin type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
Host :
Mouse
Species Reactivity:
Human, Mouse, Rat
Specificity :
Recognizes endogenous levels of Cytokeratin 18 protein.
Predicted Reactivity:
Applications :
WB 1:1000 – 1:3000, IF:IC 1:100 – 1:200
Immunogen:
Recombinant protein corresponding to human Cytokeratin 18.
Concentration :
Purification :
Clonality:
Monoclonal Antibody
Storage Temp.:
Shipped at 4 ° C Upon delivery aliquot and store at -20 ° C for one yearAvoid freeze/that cycles
Research areas :
Background :
UniProt :
P05783
Additional information:
Product Details FAQ Citations(0) Video Pictures Documents |Overview |Description Mouse monoclonal antibody to Cytokeratin 18 |Synonym CYK18; Keratin type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18 |Host Mouse |Specificity Recognizes endogenous levels of Cytokeratin 18 protein. |Species Reactivity Human, Mouse, Rat |Applications WB 1:1000 – 1:3000, IF:IC 1:100 – 1:200 |Immunogen Recombinant protein corresponding to human Cytokeratin 18. |Properties |Clonality Monoclonal Antibody |Storage Temp. Shipped at 4 ° C Upon delivery aliquot and store at -20 ° C for one yearAvoid freeze/that cycles |Target |UniProt P05783 | Western blot analysis of Cytokeratin 18 expression in HepG2 (A), Hela (B), mouse liver (C), mouse skeletal muscle (D), C2C12 (E), rat heart (F) whole cell lysates.| Immunofluorescent analysis of Cytokeratin 18 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |Tips:This product is for research use only. Not for use in diagnostic prodcedures.
Mouse anti-Cytokeratin 18 Monoclonal Antibody
Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.Related websites: https://www.medchemexpress.com/antibodies.html
2340388-72-7 Protocol 1032568-63-0 In Vivo PMID:28771291 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
