Cells were being then both stimulated with ionomycin (50 mg/mL) for 30 minutes and analyzed for ERK phosphorylation by Western blot, or stimulated with ionomycin overnight in triplicate and supernatants have been collected and analyzed for IL-2 manufacturing by ELISA
The system of energetic Ras-mediated IL-2 costimulation mimics the mechanism of CD28-mediated costimulation. A....