Murine entire-length RGSZ2 (NM_019958) and its RGS box ended up cloned by PCR from PAG cDNA. Glutathione S-transferase (GST) fusion proteins had been acquired by cloning mRGSZ2 and its RGS box into the pFN2A (GST) Flexi vector (Promega)
The vector was launched into E. coli BL21 (KRX, Promega, #L3002) and clones had...